For a long time, researchers have been working on developing therapeutic agents so that they can tailor pharmacological and pharmacokinetic properties to successfully treat diseases. While conventional drugs are widely used, the development of therapeutic nanoparticles has been radically changing the future of drug formulation and delivery, with nanoparticles becoming more popular due to their unique tunable physicochemical properties. They have shown promising results for the delivery of a variety of molecules, improving their efficacy and/or increasing their tolerability in the body. Nano-carriers may also improve the bioavailability of water-insoluble drugs, carry large payloads, protect the therapeutic agents from physiological barriers, as well as enable the development of novel classes of bioactive macromolecules. However the accurate and precise measurement of these substances within the biological matrix or cell structure is non-trivial; and often pushes high resolution analytical techniques to their limits of resolution and/or detection. In this one day workshop we plan to explore the advances that have been made in the metrology applied to analyse such nanoparticles being targeted for the next generation of drug formulation and delivery in biological systems.